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Image Search Results
Journal: bioRxiv
Article Title: Synergism of TNF-α and IFN-γ triggers inflammatory cell death, tissue damage, and mortality in SARS-CoV-2 infection and cytokine shock syndromes
doi: 10.1101/2020.10.29.361048
Figure Lengend Snippet: (A) Heatmap depicting the levels of pro-inflammatory cytokines in serum of patients with COVID-19 and healthy people . (B) Pro-inflammatory cytokines released from PBMCs infected with SARS-CoV-2. (C) Percent of BMDMs that are dead 48 h after cytokine treatment using the IncuCyte imaging system and propidium iodide (PI) staining. “Cocktail-1” contained all 8 cytokines (IL-6, IL-18, IFN-γ, IL-15, TNF-α, IL-1α, IL-1β, and IL-2). (D) Percent of BMDMs that are dead 48 h after treatment with the indicated combination of cytokines. (E) Real-time analysis of cell death in BMDMs treated with the indicated cytokines. (F, G) Representative images of cell death in BMDMs (F) and THP-1 cells (G) after 48 h of the indicated treatments. Scale bar, 50 μm. Data are representative of at least three independent experiments. ** P < 0.01; **** P < 0.0001. Analysis was performed using the one-way ANOVA (B–D) or the two-way ANOVA (E). Significance asterisks in C and D indicate the comparison to the media-treated control. Data are shown as mean ± SEM (B–E). See also Figure S1.
Article Snippet: The kinetics of cell death were determined using the
Techniques: Infection, Imaging, Staining, Comparison